Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H2

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Acrolein Antibodies »

Product Specifications

Immunogen

Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Acrolein Antibody (2H2) - BSA Free

Western Blot: Acrolein Antibody (2H2) [NBP2-59358]

Western Blot: Acrolein Antibody (2H2) [NBP2-59358]

Western Blot: Acrolein Antibody (2H2) [NBP2-59358] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 ug). Lane 3: Acrolein-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Acrolein Antibody (2H2) [NBP2-59358]

Immunocytochemistry/ Immunofluorescence: Acrolein Antibody (2H2) [NBP2-59358]

Immunocytochemistry/Immunofluorescence: Acrolein Antibody (2H2) [NBP2-59358] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Acrolein Antibody (2H2) [NBP2-59358]

Flow Cytometry: Acrolein Antibody (2H2) [NBP2-59358]

Flow Cytometry: Acrolein Antibody (2H2) [NBP2-59358] - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.
View 1 more images

Applications for Acrolein Antibody (2H2) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Acrolein

Alternate Names

Acrylic amide; Ethylene Carboxamide 2-Propenamide; 2-Propen-1-one; 2-propenal; Acraldehyde; Acrolein; Acrylic aldehyde

Additional Acrolein Products

Product Documents for Acrolein Antibody (2H2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Acrolein Antibody (2H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.