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ACP2 Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-15250

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NBP2-15250

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Chimpanzee (100%), Porcine (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ACP2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACP2. The exact sequence is proprietary.

Reactivity Notes

Mouse (87%), Rat (87%).

Localization

Lysosome membrane; Single-pass membrane protein; Lumenal side; Lysosome lumen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACP2 Antibody

Western Blot: ACP2 Antibody [NBP2-15250] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE Lysosomal acid phosphatase2 antibody, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: ACP2 Antibody [NBP2-15250] - Analysis of methanol-fixed HeLa, using ACP2 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ACP2 Antibody [NBP2-15250] - ACP2 antibody [N2C3] detects ACP2 protein at lysosome on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. ACP2 antibody [N2C3] dilution: 1:500.

Applications for ACP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Negative IF usage reported by customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACP2

This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

acid phosphatase 2, lysosomal, EC 3.1.3.2, LAP, lysosomal acid phosphatase

Gene Symbol

ACP2

UniProt

Additional ACP2 Products

Product Documents for ACP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.