Skip to main content

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D10

Format

BSA Free

Concentration

1 mg/ml

Product Summary for ACOT8 Antibody (4D10) - BSA Free

Immunogen

Recombinant human ACOT8 (1-319aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ACOT8 Antibody (4D10) - BSA Free

Western Blot: ACOT8 Antibody (4D10) [NBP2-59419] - Lane 1: Recombinant Human ACOT8, Lane 2: Ramos cell lysates, Lane 3: A431 cell lysates, Lane 4: 293T cell lysates, Lane 5: A549 cell lysates
Immunocytochemistry/Immunofluorescence: ACOT8 Antibody (4D10) [NBP2-59419] - Analysis of ACOT8 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for ACOT8 Antibody (4D10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1029

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACOT8

ACOT8 is encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACTEIII, acyl-CoA thioesterase 8HNAACTE, Choloyl-coenzyme A thioesterase, EC 3.1.2.27, hACTEIII, HIV-Nef-associated acyl-CoA thioesterase, hTEcholoyl-CoA hydrolase, long-chain fatty-acyl-CoA hydrolase, palmitoyl-CoA hydrolase, peroxisomal acyl-CoA thioesterase, peroxisomal acyl-CoA thioesterase 1, Peroxisomal acyl-coenzyme A thioester hydrolase 1, Peroxisomal long-chain acyl-CoA thioesterase 1, PTE-1, PTE1HIV-Nef associated acyl-CoA thioesterase, PTE2, PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8, Thioesterase II, thioesterase III

Gene Symbol

ACOT8

Additional ACOT8 Products

Product Documents for ACOT8 Antibody (4D10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACOT8 Antibody (4D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...