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Aconitate hydratase B Antibody (4C2B3), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-06090

Key Product Details

Species Reactivity

Validated:

E. coli

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C2B3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aconitate hydratase B Antibody (4C2B3)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant E. coli Aconitate hydratase B (Uniprot#: P36683; Met 1-Val 865).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for Aconitate hydratase B Antibody (4C2B3)

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:500-1:1000
Application Notes
Western Blot: Using a DAB detection system, the detection limit for E.coli Aconitate hydratase B is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aconitate hydratase B

Alternate Names

acnB, hypothetical protein, yacI, yacJ

Additional Aconitate hydratase B Products

Product Documents for Aconitate hydratase B Antibody (4C2B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aconitate hydratase B Antibody (4C2B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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