Acetyl-Lysine Antibody (3P8N3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15483

Novus Biologicals, part of Bio-Techne
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NBP3-15483
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3P8N3

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Acetyl-Lysine Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein corresponding to a sequence containing acetylated K.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Acetyl-Lysine Antibody (3P8N3) - BSA Free

Western Blot: Acetyl-Lysine Antibody (3P8N3)BSA Free [NBP3-15483]

Western Blot: Acetyl-Lysine Antibody (3P8N3)BSA Free [NBP3-15483]

Western Blot: Acetyl-Lysine Antibody (3P8N3) [NBP3-15483] - Western blot analysis of extracts of various cell lines, using Acetyl-Lysine antibody (NBP3-15483) at 1:1000 dilution.HeLa NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Acetyl-Lysine Antibody (3P8N3) - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (3P8N3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Acetyl-Lysine Antibody (3P8N3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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