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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A9D5E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for ACBD6 Antibody (4A9D5E9)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACBD6 (Accession#: NP_115736.1; Met 1-Ala 282). .

Reactivity Notes

No cross-reactivity in ELISA with:

Insect cell lysate

Specificity

No cross-reactivity in ELISA with: Insect cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ACBD6 Antibody (4A9D5E9)

Western Blot: ACBD6 Antibody (4A9D5E9) [NBP3-06614] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDa Observed band size:35 kDa
Immunoprecipitation: ACBD6 Antibody (4A9D5E9) [NBP3-06614] - Lane A:0.5 mg hela Whole Cell Lysate 0.5 uL anti-ACBD6 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ACBD6 mouse monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 31 kDa

Applications for ACBD6 Antibody (4A9D5E9)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000
Application Notes
This antibody will detect Virus ACBD6 in ELISA pair set (Catalog: # NBP2-79347). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP3-06598).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACBD6

ACBD6 binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1-CoA, lower affinityfor unsaturated C20:4-CoA, and very weak affinity for saturated C16:0-CoA. Does not bind fatty acids

Alternate Names

acyl-CoA binding domain containing 6, acyl-CoA-binding domain-containing protein 6, acyl-Coenzyme A binding domain containing 6, MGC2404

Gene Symbol

ACBD6

Additional ACBD6 Products

Product Documents for ACBD6 Antibody (4A9D5E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACBD6 Antibody (4A9D5E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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