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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for ABCE1 Antibody - BSA Free

Immunogen

A fusion protein containing amino acids 1-119 of human ABCE1 [UniProt# P61221]

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 25227463).

Localization

Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCE1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.
Immunocytochemistry/Immunofluorescence: ABCE1 Antibody [NB400-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ABCE1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for ABCE1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunoprecipitation

1:1000

Western Blot

1:250-1:1000
Application Notes
This ABCE1 antibody is useful for Immunoprecipitaton, Immunocytochemistry/Immunofluorescence, and Western Blot. In ICC/IF, mitochondrial staining was observed in HeLa cells. In Western Blot, a band was seen ~68 kD representing ABCE1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using NB400-116 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ABCE1

ABCE1 (ATP-binding cassette sub-family E member 1), also named RLI or HP68, belongs to ABC transporter superfamily/ABCE family and is composed of two nucleotide binding domains and an N-terminal Fe-S binding domain. ABCE1 heterodimerizes with RNASEL leading to its inhibition and has the ability to associates with HIV-1 Vif, HIV-1, HIV-2 and SIV Gag proteins. Evolutionarily highly conserved, ABCE1 localize to cytoplasm/mitochondrion as well as to virus formation clusters at plasma membrane and its highest expression has been observed in brain, kidney, prostate etc. where it gets activated by HIV-1 or EMCV (encephalomyocarditis virus). Originally identified as an RNase L inhibitor, ABCE1 has now been implicated in more fundamental processes of translation and ribosome biogenesis, and can regulate functions including viral infection/assembly of immature HIV-1 capsids, tumor cell proliferation, anti-apoptosis and is also a candidate for tumor-suppressor protein.

Alternate Names

2'-5'-oligoadenylate-binding protein, ATP-binding cassette, sub-family E (OABP), member 1, huHP68, OABP, Ribonuclease 4 inhibitor, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor, RLIABC38, RNase L inhibitor, RNASEL1, RNASELIRNS4IATP-binding cassette sub-family E member 1

Gene Symbol

ABCE1

Additional ABCE1 Products

Product Documents for ABCE1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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