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ABCB5 Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-93365

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NB100-93365

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for ABCB5 Antibody

Immunogen

Peptide with sequence C-QTQHRNTSKKAQ corresponding to internal region according to NP_848654.3, NP_001157413.1.

Epitope

C-QTQHRNTSKKAQ

Specificity

This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCB5 Antibody

Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for ABCB5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
FLOW successful usage on Hep3B cells is reported in scientific literature (PMID: 20682318) and tested in HepG2 Cells
WB: Approx. 80 kDa band observed in lysates of A431(calculated MW of 89.8 kDa band according to NP_848654.3). Primary incubation was 1 hour.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCB5

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM]

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5

Alternate Names

ABCB5 P-gp, ABCB5alpha, ABCB5beta, ATP-binding cassette protein, ATP-binding cassette sub-family B member 5, ATP-binding cassette, sub-family B (MDR/TAP), member 5, EC 3.6.3, EC 3.6.3.44, EST422562, P-glycoprotein ABCB5

Entrez Gene IDs

340273 (Human)

Gene Symbol

ABCB5

UniProt

Additional ABCB5 Products

Product Documents for ABCB5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCB5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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