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ABCA2 Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP1-20863

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NBP1-20863

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for ABCA2 Antibody

Immunogen

Peptide with sequence C-KKQSDNLEQQETEP corresponding to internal region according to NP_997698.1.

Epitope

C terminus

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Specificity

This antibody is expected to recognize both reported isoforms (NP_001597.2; NP_997698.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ABCA2 Antibody

Immunohistochemistry-Paraffin: ABCA2 Antibody [NBP1-20863] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ABCA2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

0.3 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to .NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cortex). Use in immunoprecipitation reported in scientific literature (PMID:31334335).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using NBP1-20863 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCA2

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ABC2ATP-binding cassette, sub-family A, member 2, ATP-binding cassette 2, ATP-binding cassette sub-family A member 2, ATP-binding cassette transporter 2, ATP-binding cassette, sub-family A (ABC1), member 2, EC 3.6.3, EC 3.6.3.41, KIAA1062, MGC129761

Entrez Gene IDs

20 (Human); 79248 (Rat)

Gene Symbol

ABCA2

Additional ABCA2 Products

Product Documents for ABCA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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