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ABCA1 Antibody (5A1-1422.11) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB400-165

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 5A1-1422.11

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for ABCA1 Antibody (5A1-1422.11) - BSA Free

Immunogen

Mouse ABCA1 Antibody (5A1-1422.11) expressing HeLa stable transfectants. [UniProt# P41233]

Localization

Cell Membrane

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for ABCA1 Antibody (5A1-1422.11) - BSA Free

Flow Cytometry: ABCA1 Antibody (5A1-1422.11) [NB400-165] - Unpurified [NB400-165] - Analysis of Raw (red) and Raw-cAMP (blue) cells using NB400-165 at a dilution of 1:4000

Applications for ABCA1 Antibody (5A1-1422.11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:1000 - 1:3000
Application Notes
The antibody is not reactive in Western Blot or ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NB400-165 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ABCA1

ATP-binding cassette transporter A1 (ABCA1) is a cAMP-dependent and sulfonylurea-sensitive anion transporter belonging to the ATP-binding cassette family. ABCA1 is involved in the regulation of apolipoprotein AI (apoAI)-mediated cholesterol efflux and high-density lipoproteins (HDL) metabolism (1). In the brain, ABCA1 transports cholesterol to apoE, the major CNS apolipoprotein, where it influences motor function and synaptic morphology.

ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).

References

1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617

2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522

3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574

Long Name

ATP-binding Cassette, Sub-family A (ABC1), Member 1

Alternate Names

ABC1, CERP, HDLDT1, TGD

Entrez Gene IDs

19 (Human); 11303 (Mouse)

Gene Symbol

ABCA1

Additional ABCA1 Products

Product Documents for ABCA1 Antibody (5A1-1422.11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABCA1 Antibody (5A1-1422.11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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