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AATF Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-55324

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NB100-55324

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for AATF Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-322, NB100-97831, NB600-233

Scientific Data Images for AATF Antibody

Western Blot: AATF Antibody [NB100-55324] - Detection of Human AATF/Che-1 on HeLa whole cell lysates using NB100-55324. AATF/Che-1 was also immunoprecipitated by rabbit anti-AATF/Che-1 antibody NB100-55325.

Applications for AATF Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AATF

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Alternate Names

apoptosis antagonizing transcription factor, Apoptosis-antagonizing transcription factor, CHE-1, CHE1Rb-binding protein Che-1, DEDprotein AATF

Entrez Gene IDs

26574 (Human); 56321 (Mouse)

Gene Symbol

AATF

UniProt

Additional AATF Products

Product Documents for AATF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AATF Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-322, NB100-97831, NB600-233

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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