AARSD1 Antibody (012) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90276
Recombinant Monoclonal Antibody
![Western Blot: AARSD1 Antibody (012) [NBP2-90276]](https://resources.bio-techne.com/images/products/AARSD1-Antibody-012-Western-Blot-NBP2-90276-img0001.jpg "Western Blot: AARSD1 Antibody (012) [NBP2-90276]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 012
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Uniprot#: Q9BTE6-2; Thr176-Glu525).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for AARSD1 Antibody (012) - Azide and BSA Free
Western Blot: AARSD1 Antibody (012) [NBP2-90276]
Western Blot: AARSD1 Antibody (012) [NBP2-90276] - Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 50 kDa.
Immunocytochemistry/ Immunofluorescence: AARSD1 Antibody (012) [NBP2-90276] -
Immunocytochemistry/ Immunofluorescence: AARSD1 Antibody (012) [NBP2-90276] - Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90276 (1:60) at 4 overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.Applications for AARSD1 Antibody (012) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AARSD1
Alternate Names
alanyl-tRNA editing protein Aarsd1, alanyl-tRNA synthetase domain containing 1, Alanyl-tRNA synthetase domain-containing protein 1, MGC2744
Gene Symbol
AARSD1
Additional AARSD1 Products
Product Documents for AARSD1 Antibody (012) - Azide and BSA Free
Product Specific Notices for AARSD1 Antibody (012) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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