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AADACL1 Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-76805

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NBP2-76805

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for AADACL1 Antibody

Immunogen

Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3; NP_001139748.2; NP_001139749.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AADACL1 Antibody

Flow Cytometry: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Applications for AADACL1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunofluorescence

10 ug/ml

Peptide ELISA

Detection Limit 1:1000
Application Notes
WB: Preliminary experiments gave an approx. 26-28 kDa band in human brain (amygdala, cerebellum, hippocampus) lysates after 1 ug/ml antibody staining. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1 kDa band according to NP_065843.3 and 31.2 kDa according to NP_001139749.1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AADACL1

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.

Long Name

Neutral cholesterol ester hydrolase 1

Alternate Names

EC 3.1.1.-, EC 3.1.1.71, KIAA1363, NCEH, NCEH1

Entrez Gene IDs

57552 (Human); 320024 (Mouse)

Gene Symbol

NCEH1

Additional AADACL1 Products

Product Documents for AADACL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AADACL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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