5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45121

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Inhibition Activity

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 1E9

Format

BSA Free

Concentration

1 mg/ml

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

Highly purified human placental 5'-Nucleotidase/CD73 (Uniprot#: P21589)

Reactivity Notes

This antibody does not show cross reactivity with mouse 5'-Nucleotidase/CD73

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] -

Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] - Immunofluorescence analysis of paraformaldehyde-fixed U251 cells on coverslips stained with NBP3-45121 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic, nuclear, and nuclear bodies staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45121, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] -

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free [NBP3-45121] - Paraformaldehyde-fixed U251 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (negative control, black line) or NBP3-45121 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Applications for 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Inhibition Activity

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (1E9) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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