5-MethylCytosine Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-744
![Western Blot: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0005.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744]")
![Western Blot: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0006.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat, All Species
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
5-Methyl cytosine conjugated to KLH.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 20808872).
Specificity
This is specific for 5-Methyl Cytosine. It was tested using immunoprecipitation against 5-Methyl Cytosine and bromodeoxyuridine (BrdU) or a control (no antigen).
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for 5-MethylCytosine Antibody - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/Cytosine-5-Methyl-Antibody-Immunocytochemistry-Immunofluorescence-NB100-744-img0004.jpg "Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]")
Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]
Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.Applications for 5-MethylCytosine Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
25-100 ug/ml
Application Notes
Western blot images and application from PMID 20808872.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.5)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine Antibody - Azide and BSA Free
Product Specific Notices for 5-MethylCytosine Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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