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5-Formylcytosines Antibody (RM477), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-18864

Recombinant Monoclonal Antibody
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NBP3-18864

Key Product Details

Species Reactivity

Validated:

Vertebrate

Applications

Dot Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM477

Concentration

1 mg/ml

Product Summary for 5-Formylcytosines Antibody (RM477)

Immunogen

KLH-conjugated 5-Formylcytosines

Specificity

This antibody reacts to 5-Formylcytosines in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-Formylcytosines Antibody (RM477)

5-Formylcytosines Antibody (RM477)

Dot Blot: 5-Formylcytosines Antibody (RM477)[NBP3-18864]

Dot Blot: 5-Formylcytosines Antibody (RM477)[NBP3-18864]Dot blot of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).

Applications for 5-Formylcytosines Antibody (RM477)

Application
Recommended Usage

Dot Blot

0.5 - 2 ug/ml

ELISA

0.5 - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: 5-Formylcytosines

Additional 5-Formylcytosines Products

Product Documents for 5-Formylcytosines Antibody (RM477)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-Formylcytosines Antibody (RM477)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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