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15-PGDH/HPGD Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-1482

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NB100-1482

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for 15-PGDH/HPGD Antibody

Immunogen

Peptide with sequence C-DYDTTPFQAKTQ corresponding to C-Terminus according to NP_000851.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 15-PGDH/HPGD Antibody

Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Liver. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for 15-PGDH/HPGD Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 1 ug/mL
Application Notes
WB: Experiments gave bands at approx. 26 kDa band and 50 kDa band in human duodenum lysates after antibody staining. These bands correspond to earlier findings in scientific literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0 kDa band according to NP_000851.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 15-PGDH/HPGD

Prostaglandins are involved in many physiologic and cellular processes, such as inflammation. The NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH, type I) is the main enzyme of prostaglandin degradation. By catalyzing the conversion of the 15-hydroxyl group of prostaglandins into a keto group, this ubiquitous enzyme strongly reduces the biologic activity of these molecules. The induction of 15-PGDH expression by dexamethasone and other glucocorticoids at the therapeutic level provides an additional biochemical mechanism for the anti-inflammatory action of these glucocorticoids.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Entrez Gene IDs

3248 (Human)

Gene Symbol

HPGD

UniProt

Additional 15-PGDH/HPGD Products

Product Documents for 15-PGDH/HPGD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 15-PGDH/HPGD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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