14-3-3 zeta/beta Antibody (23GB4830)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50373
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB4830
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human 14-3-3 zeta/beta (Uniprot: P31946/P63104)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 14-3-3 zeta/beta Antibody (23GB4830)
Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/beta Antibody (23GB4830)[NBP3-50373] -
Immunocytochemical staining of HepG2 cells with 14-3-3 zeta/beta antibody (1:1,000). Nuclei were stained blue with DAPI; 14-3-3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Flow Cytometry: 14-3-3 zeta/beta Antibody (23GB4830)[NBP3-50373] -
Flow cytometric analysis of 14-3-3 zeta/betaexpression in HepG2 cells using 14-3-3 zeta/beta antibody (1:2,000). Green, isotype control; red, 14-3-3.
Western Blot: 14-3-3 zeta/beta Antibody (23GB4830)[NBP3-50373] -
Western blotting analysis using 14-3-3 zeta/beta antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for 14-3-3 zeta/beta Antibody (23GB4830)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 14-3-3 zeta/beta
Alternate Names
KCIP-1MGC126532,14-3-3-zeta, MGC111427, MGC138156, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide, zeta polypeptide
Gene Symbol
YWHAZ
Additional 14-3-3 zeta/beta Products
Product Documents for 14-3-3 zeta/beta Antibody (23GB4830)
Product Specific Notices for 14-3-3 zeta/beta Antibody (23GB4830)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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