14-3-3 epsilon Antibody (24GB2120)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50616

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB2120

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 epsilon Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human 14-3-3 epsilon (Uniprot: P62258)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 14-3-3 epsilon Antibody (24GB2120)

14-3-3 epsilon Antibody (24GB2120)

Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody (24GB2120)[NBP3-50616] -

Immunocytochemical staining of HepG2 cells with 14-3-3 epsilon antibody (20 um.
14-3-3 epsilon Antibody (24GB2120)

Flow Cytometry: 14-3-3 epsilon Antibody (24GB2120)[NBP3-50616] -

Flow cytometric analysis of 14-3-3 epsilonexpression in HepG2 cells using 14-3-3 epsilon antibody (1:2,000). Green, isotype control; red, YWHAE.
14-3-3 epsilon Antibody (24GB2120)

Western Blot: 14-3-3 epsilon Antibody (24GB2120)[NBP3-50616] -

Western blotting analysis using 14-3-3 epsilon antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for 14-3-3 epsilon Antibody (24GB2120)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody (24GB2120)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 14-3-3 epsilon Antibody (24GB2120)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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