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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 02

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon (Accession#: NP_006752.1; Met1-Gln255).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

14-3-3 epsilon Antibody (02) - Azide and BSA Free

Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] -

Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa
14-3-3 epsilon Antibody (02) - Azide and BSA Free

Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] -

Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate 4 uL NBP3-26078 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26078, at 1:100 dilution. Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2 [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa

Applications for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 epsilon Antibody (02) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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