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14-3-3 Antibody (SR1456), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-21695

Recombinant Monoclonal Antibody
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NBP3-21695-100ul
NBP3-21695-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1456

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 14-3-3 Antibody (SR1456)

Immunogen

A synthesized peptide derived from human 14-3-3 (Uniprot #: P31946, P63104)

Specificity

Detects endogenous levels of 14-3-3

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 Antibody (SR1456)

Western Blot: 14-3-3 Antibody (SR1456) [NBP3-21695] -

Western Blot: 14-3-3 Antibody (SR1456) [NBP3-21695] - Western blot analysis using NBP3-21695 in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate.

Applications for 14-3-3 Antibody (SR1456)

Application
Recommended Usage

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Alternate Names

1433

Gene Symbol

YWHAQ

Additional 14-3-3 Products

Product Documents for 14-3-3 Antibody (SR1456)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 Antibody (SR1456)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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