Skip to main content

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-24484

Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-24484

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, urine, cell samples, cell culture supernatant and tissue

Sensitivity

1.35 U/mL

Assay Range

1.35 - 62 U/mL

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (530 - 570 nm, optimum wavelength: 550 nm)
Assay time: 70 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.1

Inter-Assay Precision (Precision between assays) %CV < 9.6

Recovery for SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 99%

Scientific Data Images for SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] -

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Standard Reference Curve
SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] -

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Assay Example
SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] -

SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Assay Example

Kit Contents for SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Nitrosogenic Agent
  • Reagent 3: Substrate Solution
  • Reagent 4: Enzyme Stock Solution
  • Reagent 5: Enzyme Diluent
  • Reagent 6: Chromogenic Agent A
  • Reagent 7: Chromogenic Agent B
  • Reagent 8: Chromogenic Agent C
  • Reagent 9: Extracting Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SOD1/Cu-Zn SOD

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

Additional SOD1/Cu-Zn SOD Products

Product Documents for SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Loading...
Loading...