HT gamma-H2AX Pharmacodynamic Assay
R&D Systems, part of Bio-Techne | Catalog # 4418-096-K
For the quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.
Key Product Details
Features
Enables the quatification of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies.
Key Benefits
- Chemiluminescent, non-radioactive ELISA
- Pre-coated 96 well capture antibody plate
- Dynamic range from 10 pM to 800pM
- Sensitivity with 5 pM of gamma-H2AX
Species
Multi-Species
Product Summary for HT gamma-H2AX Pharmacodynamic Assay
Product Specifications
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.
Other Resources
Kit Contents for HT gamma-H2AX Pharmacodynamic Assay
- Gamma-H2AX Standard, 1 µM
- Jurkat Cell Lysate Control
- Assay Buffer
- Cell Lysis Reagent
- 25X Wash Buffer
- 96-Stripwell pre-coated plate with 3 film sealers
- PARP PeroxyGlow A
- PARP PeroxyGlow B
- H2AX IgM Detecting Antibody
- Goat anti-Mouse IgM HRP Conjugate
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Additional Histone H2AX Products
Product Documents for HT gamma-H2AX Pharmacodynamic Assay
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