Autophagy/Cytotoxicity Dual Staining Kit
Novus Biologicals, part of Bio-Techne | Catalog # KA1299
Key Product Details
Species
Insect
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry/ Immunofluorescence
Kit Type
Kit
Product Summary for Autophagy/Cytotoxicity Dual Staining Kit
Autophagy/Cytotoxicity Dual Staining Kit is a fluorescence-labeled method for screening autophagy and cytotoxicity at the cellular level.
Product Specifications
Sample Volume
5 x 10^4 cells per well. All Treatments conditions should be assayed in duplicate at minimum.
Reactivity Notes
Reacts with Frugiperda.
Scientific Data Images for Autophagy/Cytotoxicity Dual Staining Kit
Functional: Autophagy/Cytotoxicity Dual Staining Kit [KA1299]
Functional: Autophagy/Cytotoxicity Dual Staining Other Kit [KA1299] - Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy. Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles. Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide. Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen. Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicleKit Contents for Autophagy/Cytotoxicity Dual Staining Kit
- Cell-Based Assay Buffer Tablet
- Cell-Based Monodansylcadaverine
- Cell-Based Propidium Iodide Solution
- Cell-Based Tamoxifen (100 mM)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage of components varies. See protocol for specific instructions.
Background: Autophagy/Cytotoxicity Dual Staining
Additional Autophagy/Cytotoxicity Dual Staining Products
Product Documents for Autophagy/Cytotoxicity Dual Staining Kit
Product Specific Notices for Autophagy/Cytotoxicity Dual Staining Kit
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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