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Acetylcholinesterase/ACHE Assay Kit (Fluorometric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # KA4131

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KA4131

Key Product Details

Sample Type & Volume Required Per Well

Biological Samples (50 ul)

Sensitivity

0.01 mU

Assay Range

0.1 mU/ml to 100 mU/ml

Product Specifications

Assay Type

Fluorometric

Kit Type

Assay Kit (Fluorometric)

Specificity

Acetylcholinesterase Assay Kit (Green Fluorescence) is a one-step fluorometric assay for the detection of AChE activity. Excitation (Max): 490 nm, Emission (Max): 520 nm.

Description

Quality control test: Standard curve This kit uses our outstanding Thiolite Green to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. Thiolite Green is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument.

Scientific Data Images for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Acetylcholinesterase/ACHE Assay Kit (Fluorometric) [KA4131] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

  • Acetylcholinesterase Standard (5 units)
  • Acetylthiocholine
  • Assay Buffer
  • DMSO
  • Thiolite Green

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Acetylcholinesterase/ACHE

Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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