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Janelia Fluor® Dyes for Super-Resolution Microscopy

Developed at the Janelia Research Campus, Janelia Fluor® dyes are exceptionally bright and highly photostable. They are well suited for super-resolution techniques including STED, PALM, and dSTORM.

Janelia Fluor® dyes are supplied with a range of reactive groups for biomolecular conjugation, including:

Conjugation protocols are available on

All amine reactive dyes can be converted to relevant substrates for use in self-labeling tag systems such as HaloTag® and SNAP-tag®.


All images kindly provided by Professor. C. Soeller, University of Exeter; images taken by Alex Clowsley and Anna Meletiou.

SNAP-tag is a trademark of New England BioLabs, Inc and HaloTag is a trademark of Promega Corporation.

Cardiac Tissue labelled with Janelia Fluor® Dyes. Images acquired using dSTORM
Photoactivatable Janelia Fluor® Dyes

Photoactivatable Janelia Fluor® Dyes

Photoactivatable Janelia Fluor® dyes are a favourable alternative to photoactivatable proteins for PALM microscopy. PA Janelia Fluor® 646, SE (Cat. No. 6150) and PA Janelia Fluor® 549, SE (Cat. No. 6149) can be used on both lived and fixed cells and can be multiplexed to perform two-colour sptPALM in live cells. Individually these dyes are suitable for single molecule tracking and PALM and sptPALM.

HM Janelia Fluor® 526, HM JF526

Featured dye: HM Janelia Fluor® 526

HM Janelia Fluor® 526, SE, (Cat. No. 7312) is a spontaneously blinking green-emitting dye, which is ideal for STED microscopy, single-molecule localization spectroscopy (SMLSM) and dSTORM. The spontaneous blinking properties of HM Janelia Fluor® 526, SE avoids the need for using strongly reducing dSTORM buffers.



Bio-Techne brands R&D Systems and Novus Biologicals, offer 780,000 antibodies which have been validated in over 25 species for more than 15 applications.

Super-Resolution Microscopy Types and Tips

Super-Resolution Microscopy Resources

Fluorescent Probes and Dyes Brochure from Tocris

Fluorescent Probes and Dyes Brochure

This product guide showcases our range and gives background on the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals

Super-Resolution Microscopy References