UBE2N (Ubc13)/Uev1a Complex: Proteins and Enzymes

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein. Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a Complex functions with Ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked Ubiquitin chains. These Ubiquitin chains can either be unanchored or attached to target proteins. The UBE2N (Ubc13)/Uev1a Complex localizes primarily to the cytoplasm and is important for NF-kappa B activation. Additionally, this complex may function in the intracellular response to retroviral capsid recognition. Pathologically, the UBE2N (Ubc13)/Uev1a Complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer.