Skip to main content

Upcoming Webinars

Talk Title and Presenter(s)

Date and Time

Breaking Through the Challenges of rAAV Purity Characterization by CE-SDS

Ana Carreras González, PhD. 

View Details | Register

14 June

10.30am BST / 11.30am CEST

On-Demand

Talk Title and Presenter(s)

Date Aired

Unlocking the potential of Long-COVID-19: Developing a Diagnostic Test

Alexandre Lucas, PhD and Benjamin Charvet, PhD.

Watch Now

20 April

Breaking Through the Challenges of rAAV Purity Characterization by CE-SDS

14 June, 10.30 am BST / 11.30 am CEST

Despite recent advances in the gene therapy field, significant challenges lie ahead for the consistent manufacturing and release of these medicines. rAAV particles are built from the folding of three major capsid proteins, VP1, VP2, and VP3, harbouring the therapeutic gene of interest. These proteins present a theoretical ratio of 1:1:10, migrating at approximately 87, 73, and 62 kDa, respectively.

To characterize rAAV purity, CE-SDS offers a state-of-the-art approach with high resolution and sensitivity and providing semi-quantitative estimate in terms of % Purity of the samples. Nevertheless, several challenges have been faced when transferring the purity assessment from SDS-PAGE to CE-SDS in terms of salt content, virus stability and impurity-nature characterization. In this webinar the different issues and the achieved solutions faced during rAAV purity characterization by CE-SDS will be discussed.

Presenters: Ana Carreras González, PhD. 

Instrument user webinar

Speaker

Assessing Purity of rAAV by CE-SDS

Ana Carreras González, PhD

QC Biochemistry Department Scientist at Viralgen Vector Core Company

Dr. Ana Carreras joined Viralgen Vector Core almost three years ago, as a member of the Quality Control department. This company stands as a leading CDMO in the field of rAAV manufacturing for gene therapy and innovative medicines. As a member of QC team, Ana was involved in several projects, leading the set-up of different techniques to perform the characterization of rAAV for product release. She developed product purity assessment by iCE, product identity by WESS and impurity quantification by automated immunoassay.

Come to Our Next Instrument User Meeting

Join us in Dublin for Analytical Exchange 2023: a free two-day event designed for and led by instrument users looking to network and share best practices to optimize their automated protein analysis capabilities.

Join Us at Our Next European Instrument User Meeting

Want to Present an Upcoming Webinar? Get in Touch