Biotinylated proteins can be powerful tools for assessing protein interactions and screening antibody or small molecule libraries for potential therapeutics. R&D Systems™ Avi-tag biotinylated proteins contain an extra 15 amino acid sequence, the Avi-tag, fused to the N- or the C-terminus of the protein, which is enzymatically biotinylated by the E. coli BirA biotin ligase on a single lysine residue. In contrast to amine biotinylation, Avi-tag biotinylation is highly specific, consistent, and generates a homogeneous product with uniform protein orientation when bound to a streptavidin-coated surface.
Read this application note to learn how R&D Systems Avi-tag biotinylated proteins can be used as an alternative to chemical or amine biotinylated proteins to determine protein interaction kinetics and affinity using the Biacore surface plasmon resonance system.
