SITC 2023 Poster: A Novel Protease-Free Method for the Co-Detection of RNA and Protein Biomarkers using the RNAscope™ Technology

The protease-free RNAscope co-detection workflow will serve as a powerful multi-omics staining technique for a wider range of antibodies by enabling visualization of RNA-protein co-detection for the comprehensive profiling of tissue microenvironments, facilitating faster breakthroughs in the discovery of therapeutics.

We present results using protease-free reagent in a variety of different IHC and ISH-IHC co-detection assays on Leica and Ventana autostaining platforms, suggesting compatibility with different RNAscope offerings and resulting in complete protein restoration with negligible impact to RNA while maintaining tissue integrity.