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Novel icIEF Fractionation Coupled with Different MS Systems for Rapid Charge Variant Characterization of Therapeutic Antibodies

by ProteinSimple, a Bio-Techne brand.

Scientific Meeting Posters

Scientific Meeting Posters Summary

icIEF Fractionation Coupled with Mass Spec for Charge Characterization of Therapeutic Antibodies Poster

Characterizing the charge variant species in a biotherapeutic molecule is critical for successful BLA submission. This poster describes a novel icIEF fractionation solution, which involves icIEF separation and the collection of icIEF-resolved charge variants of an ADC’s parent mAb using a fractionation cartridge on the new MauriceFlex™ system. The poster highlights:

  • MauriceFlex icIEF-based peak fractionation and mass spectrometry characterization of fractions
  • Successful fraction collection in less than two hours with 80 - 100% purity using icIEF separation conditions with urea
  • Characterization on both ZipChip-MS and LC-MS platforms using fractions from a single fractionation run
  • Intact and peptide mapping of charge variants
  • Key advantages of using icIEF-based fractionation on MauriceFlex

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