Summary
The new RNA-protein Co-detection workflow improves IHC/IF antibody compatibility with the RNAscope ISH technology creating an extremely powerful multiomic solution to elucidate cellular heterogeneity, identify novel cell populations while retaining spatial information with morphological context.
- Using the RNAscope RNA protein co-detection assays we assessed the tumor microenvironment and characterized tumor infiltrated immune cells.
- We identified immune cell sub-populations using cell marker-specific antibodies and detected expression of activating chemokines and cytokines using RNAscope ISH.
- With the new Multiplex Fluorescent v2 assay with TSA Vivid™ dyes, low-abundance cytokines were visualized with increased signal intensity and resolution.
- The co-detection reagents and workflow are compatible with manual and automated platforms and can be combined with most RNAscope, BaseScope and miRNAscope assays
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