Seasonal respiratory viral pathogens can often be challenging to distinguish from one another symptomatically. For accurate and early detection of these viruses, extremely sensitive molecular detection methods like real-time PCR demand availability and inclusion of standard RNA positive controls in the test system.
- Here we describe a robust, sensitive, accurate, and cost-effective microchipbased Influenza A, Influenza B, and SARS-CoV-2 rapid detection system that utilized Armored RNA Quant®* controls (Asuragen, a Bio-Techne Brand, TX) to achieve these objectives.
- These molecular controls were also used for quality control checks of ready-to-use microchips with pre-loaded and lyophilized reagents, as positive extraction controls, and for determining technical limit of detection (LoD).
