Easy Particle Analysis for Viscous Samples with MFI

The application note discusses the use of Micro-Flow Imaging (MFI) for analyzing viscous samples in protein therapeutics development, providing precise and quantitative characterization of particle size, concentration, and morphology without the need for sample dilution.

Key Points

• MFI 5200 system accurately determines particle concentration and size in samples with viscosities up to 20 cP, without adverse impacts on morphologic parameters.
• The system consistently analyzes the same sample volume regardless of viscosity, eliminating the need for recalibrating the pump and ensuring accurate determination of particle concentration.
• Practical tips for handling viscous samples include equilibrating the flow cell, avoiding air bubbles, and ensuring proper mixing to achieve accurate sample characterization.