This poster presents an innovative workflow that intelligently and automatically correlates icIEF charge profiles to mass spectrometry data, addressing the challenges of complex icIEF-MS linking or tedious offline analysis. The workflow takes icIEF fraction data from the MauriceFlex™ system and integrates it into the Byos® platform, enabling the superimposition of icIEF and LC-MS data for a comprehensive charge variant analysis. Using a monoclonal-like antibody (NISTmAb) as a sample, the workflow highlights the discrepancies and similarities between the techniques, providing a deeper understanding of protein composition and facilitating orthogonal analysis with visual outputs.
