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Protein Aggregation and Particle Analysis

Micro-Flow MFI Instrument from ProteinSimple a Bio-Techne brand

Protein aggregation and subvisible particle analysis is a breeze with Micro-Flow Imaging (MFI). Not only is MFI more sensitive than traditional particle detection techniques, it also lets you discriminate between translucent protein aggregates and other particulates. MFI takes images of the particles in your sample, giving you more data beyond just size and count. With 10 different types of morphological data at your disposal, you'll know exactly what your particles are—silicone oil, air bubble, protein aggregate, or something else. You can't do that with standard light obscuration!

About Protein Aggregation

How Is MFI Better Than Light Obscuration For Measuring Protein Aggregates?

Reduce Recall Risk Due to Particulate Matter Contamination With MFI

Particulate matter contamination was the second highest cause of recall by the FDA in 2016. Current contamination detection methods, like Light Obscuration (LO), are limited in their ability to detect all particulate matter. Why? Protein aggregates are often highly translucent and therefore missed by compendial methods. Work done by the National Institute of Standards and Technology has shown that Light Obscuration is less sensitive to particles whose refractive index closely matches the surrounding matrix. This leads to both undercounting and under-sizing of protein aggregates.

Protein aggregation analysis using Micro-Flow Imaging (MFI) Graph

MFI Technology Combines Digital Microscopy and Microfluidics

Micro-Flow Imaging combines the direct imaging capabilities of digital microscopy with the precise control of microfluidics. Therefore, you get a complete image of what passes through the flow cell. Translucent protein aggregates that would be missed by LO are caught by MFI. From those images, morphology is derived which can then be used to differentiate particle populations within the sample. With a more sensitive detection method and the ability to separate protein from non-protein, MFI gives you the most complete profile of particles in your sample.


Figure 2 (Right). MFI’s high sensitivity allows it to detect translucent particles, like protein aggregates, that are invisible to traditional methods like Light Obscuration. Its image-based approach allows MFI to distinguish between different particle populations within a sample, including protein aggregates, silicon oil and other contaminants.

Micro-Flow Imaging Diagram

Watch This Video To Learn How MFI Gives You the Most Complete Profile of Particles in Your Sample

Seeing Beyond Light Obscuration with Micro-Flow Imaging
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