Visualize Terminal Glycans

This simple and sensitive technique uses gycoenzymes to selectively visualize terminal glycans using standard SDS-Page methods.

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Using SDS-PAGE
SDS-Page for visualizing terminal glycans, image two – visualize terminal glycans with fluorescence
This sensitive and specific technique uses glycoenzymes to selectively visualize terminal glycans in cultured cells with fluorescence.

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This publication describes a highly specific approach to visualize O-GlcNAc as well as other potential sites for O-GlcNAc modification on biological samples using recombinant glycosyltransferases and chemical probes.
This publication describes highly specific approaches for Heparan Sulfate (HS) and T/Tn antigen visualization.
This publication describes a novel Heparanase (HPSE) assay that is very sensitive and highly reproducible.
This publication describes a method for glycoprotein labeling/carbohydrate detection through glycan replacement termed glycoprotein labeling with click chemistry (GLCC).
This book chapter describes nonradioactive colorimetric assays for glycosyltransferases and sulfotransferases.
This publication describes a method for O-GlcNAc detection using in vitro sulfation using the two GlcNAc sulfotransferases carbohydrate sulfotransferase (CHST) 2 and CHST4 and the radioisotope 35S.
This publication describes a highly quantitative and reproducible nonradioactive universal phosphatase coupled sulfotransferase assay.
This publication describes a highly quantitative and reproducible nonradioactive universal phosphatase coupled glycosyltransferase assay.
This publication describes a method for detecting glycosaminoglycans (GAGs) in cells and tissue using radiolabeled in vitro sulfation with carbohydrate specific sulfotransferases.
This publication describes a novel sulfotransferase assay that uses SDS-PAGE methods.