Granulocyte Marker Antibody (SPM250) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34750

Novus Biologicals, part of Bio-Techne
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NBP2-34750-0.1mg
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Key Product Details

Species Reactivity

Human, Rhesus Macaque

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM250

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Granulocyte Marker Antibodies »

Product Specifications

Immunogen

Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.

Reactivity Notes

Macaque Monkey.

Localization

Cytoplasmic

Specificity

Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. monoclonal antibody BM-2 reacts with early precursor and mature forms of human myeloid cells. This monoclonal antibody is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Marker

Macrophage / Granulocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32856).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Granulocyte Marker Antibody (SPM250) - Azide and BSA Free

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - Azide and BSA Free [NBP2-34750]

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - Azide and BSA Free [NBP2-34750]

Immunohistochemistry-Paraffin: Granulocyte Marker Antibody (SPM250) - Azide and BSA Free [NBP2-34750] - Formalin-fixed, paraffin-embedded tonsil stained with Granulocyte MAb (SPM250). Note specific cytoplasmic staining.

Applications for Granulocyte Marker Antibody (SPM250) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-5ug/ml for coating

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Granulocyte Marker

Alternate Names

Granulocytes;PML;PMN

Additional Granulocyte Marker Products

Product Documents for Granulocyte Marker Antibody (SPM250) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Granulocyte Marker Antibody (SPM250) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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