Do you know of anyone that has used this kit for plant tissues, preferably intact tissues? Also, do you have references where people have used your ChIP kit available?

We don't know of anyone using this kit on plants at this time. H3 is very well conserved, even in plants, as the immunogen to generate the antibody shares 100% similarity with tobacco, rice, maize and most other plants. This kit will almost certainly work with your plant. The main problem is that the primers almost certainly won't work on plants. Even one base pair difference can prevent the primers from working. Chances are there is some dissimilarity at the DNA level that doesn't effect the protein sequence because of the wobble rule. You can most likely use this kit on your plant, but you will need to design your own control primers.

I am planning ChIP experiments using a rat cell line and also rat tissues. I am interested in your Chromata ChIP kit but I have some questions. The magnetic tube separator rack: is it possible to use a magnetic separator from Miltenyi Biotech (we already have one)? Is the positive control antibody (H3K4Me3) and primers (human RPL30) would work with rat samples? If no, could you provide a positive control working with the rat specie or give the primers sequences? Finally, what are the changes that have to be made to the protocol for ChIP assays starting from tissues? (Only an example using cells is given in the protocol)

1. Yes, your magnetic rack will work just fine. 2. Unfortunately, our primers do not work with species other than human and we do not have any other primers available. You should be able to design your own to the same target in rat though and it should be just fine. 3. We are in the process of launching tissue specific protocols for use in ChIP. These are not available online yet but if you contact our Technical Team we can assist you with this

I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?

As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.

I received an inquiry about ChromataChIP Kit. Regarding phase 3(p.6), what size tube should be used? And what size tubes can be used for Magnetic tube separator rack (Cat# NBP1-72032)?

Generally this step is performed using 1.5mL tubes. The stand can hold eight 1.5-ml tubes or one 15-ml tube, and one 50-ml tube.

I wanted to know whether the H3K4me3 positive control antibody has cross reactivity with other species? I am using mouse tissue and would like to know whether this antibody will still work?

The positive control antibody in this kit is expected to cross-react with mouse, rat, c. elegans and most other mammals. It will work just fine for mouse.

Regarding the ChromataChIP Kit cat.#NBP1-71709: 1. What antibodies do you have? 2. Do you supply the qPCR primers for humans (positive and negative targets for each antibody)? 3. Do you supply the negative antibody? I like your guarantee, but hope never to hold you to it!

I am glad you are interested in our ChromataChIP Kits and would be happy to answer your questions. We have several ChIP grade epigenetics antibodies available and this link should take you to additional information about the kit itself and related products. I don't know if you saw this page first before inquiring further about the kit. Let me know if so and you still had additional questions and I would be happy to address those with the lab person who developed the kit. We are proud of our guarantee and of course prefer that no one would have to use it, but sometimes that is inevitable and as long as we can resolve any issues that arise are happy to do solid completely understand and know that is where your focus and attention will be. Let us know once you have more time on your hands if you are still interested in the lab visit and I can coordinate that with the lab.

Chromata ChIP Kit (ChIP)

Name: Chromata ChIP Kit (ChIP)
Brand: Novus Biologicals
SKU: NBP1-71709
Rating: 5 (2 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71709

(2)

Citations (11)

Product Datasheet / COA / Protocols & Manuals

Discontinued Product

NBP1-71709 has been discontinued. View all Chromata ChIP products.

Product Summary
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Chromatin Immunoprecipitation

Kit Type

Kit (ChIP)

Product Summary for Chromata ChIP Kit (ChIP)

This kit contains all the necessary components to perform 30 Immunoprecipitation experiments including a positive control antibody (NB21-1023SS) and PCR primers for negative and positive controls. This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately.

Scientific Data Images for Chromata ChIP Kit (ChIP)

Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HeLa Nuclear Extracts. Image from verified customer review.

Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.

Chromatin Immunoprecipitation: Chromata ChIP Kit [NBP1-71709] - ChIP analysis of HEK293 and SH-SY5Y cells. Image from verified customer review.

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Kit Contents for Chromata ChIP Kit (ChIP)

10X Glycine (30 mL)
5 M NaCl (1 mL)
DNA Binding Buffer (18 mL)
DNA Elution Buffer (2 mL)
DNA Wash Buffer (5 mL - add 20 mL EtOH)
H3 K4Me3 Positive Control Antibody (0.025 mg) - NB21-1023SS
Human AFM Negative Control Primer Pair at 10 uM (0.5 mL) - NBP1-71653
Human RPL30 Positive Control Primer Pair at 10 uM (0.5 mL) - NBP1-71651
IP Dilution Buffer (2 x 25 mL)
IP Elution Buffer (7 mL)
IP Wash Buffer 1 (15 mL)
IP Wash Buffer 2 (15 mL)
IP Wash Buffer 3 (15 mL)
IP Wash Buffer 4 (15 mL)
Protein A/G Magnetic Beads (0.8 mL)
Proteinase K (0.06 mL)
Purification Columns (30 EA)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: Chromata ChIP

Novus' ChromataChIP Kits are a complete pack to help you perform Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer. ChromataChIP Kits come with everything you need to perform Chromatin IP including a colorful, step-by-step protocol pamphlet to walk you through the 4 main phases of ChIP. Learn more by visiting www.novusbio.com/ChromataChIP

Product Documents for Chromata ChIP Kit (ChIP)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ChromataChIP Kit Protocol NBP1-71709

COA

Supplemental Product Documentation

Download MSDS

Product Specific Notices for Chromata ChIP Kit (ChIP)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Citations
Reviews

Protocols
FAQs