VECTASTAIN(R) ABC-AMP Reagent

Novus Biologicals, part of Bio-Techne | Catalog # AK-6000-NB

Discontinued Product
AK-6000-NB has been discontinued. View all VECTASTAIN(R) ABC-AMP Reagent products.

Key Product Details

Applications

ELISA, Western Blot

Product Summary for VECTASTAIN(R) ABC-AMP Reagent

Features:

  • Amplified ABC alkaline phosphatase reagent for protein blot detection
  • Uses biotin/streptavidin technology
  • Use with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
  • Can detect as little as 1 pg target protein when used with DuoLuX™ Substrate

    • Product Specifications

    Application Notes

    The VECTASTAIN ABC-AmP Reagent is sufficient to develop approximately twenty 100 cm2 blots..

    Scientific Data Images for VECTASTAIN(R) ABC-AMP Reagent

    Western Blot: VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB]

    Western Blot: VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB]

    Western Blot: VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB] - Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane and detected with biotinylated antibody.
    VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB]

    Kit Contents for VECTASTAIN(R) ABC-AMP Reagent

    • 0.5 ml Reagent A
    • 0.5 ml Reagent B

    Formulation, Preparation, and Storage

    Concentration

    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C. Do not freeze.

    Background: VECTASTAIN(R) ABC-AMP Reagent

    Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

    The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

    The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.

    Product Documents for VECTASTAIN(R) ABC-AMP Reagent

    Product Datasheets

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for VECTASTAIN(R) ABC-AMP Reagent

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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