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pCL-Eco Retrovirus Packaging Vector

Catalog # NBP2-29540 | Novus Biologicals a Bio-Techne Brand
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NBP2-29540

Key Product Details

Species

Mouse, Rat

Applications

N/A

Product Specifications

Specificity

Ecotropic (MoMuLV) for mouse and rat cells only (not human).
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster).
10A1 (MuLV) for most mammalian cells (including hamster).

Applications

Retroviral Production (reported in scientific literature (PMID 24760698))

Application Notes

The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24760698). This product can be used in human cells to produce virions to infect non-human cells for protein production.

Scientific Data Images for pCL-Eco Retrovirus Packaging Vector

pCL-Eco Retrovirus Packaging Vector [NBP2-29540]

Formulation, Preparation, and Storage

Formulation

10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCL-Eco Retrovirus Packaging Vector

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

Alternate Names

pCLECO, pCL-Eco, pCL-Eco Retrovirus

Product Specific Notices for pCL-Eco Retrovirus Packaging Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for pCL-Eco Retrovirus Packaging Vector (102)

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FAQs for pCL-Eco Retrovirus Packaging Vector

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  • Q: I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

    A: These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

  • Q: I'm currently producing a retrovirus with the pCL-Eco packaging vector. However, my target cells are not getting infected, so I was thinking of switching to a lentivirus. Can I use this pCL-Eco packaging vector with a lentivirus, and if so, is it still only infectious to rodents?

    A: Unfortunately, we have not yet tested the pCL-Eco vector kit with lentivirus, therefore, we would not be able to provide you with any information regarding this type of vector. I am sorry for any inconvenience.

  • Q: Can I use your packaging plasmid PCL-Eco Retrovirus packaging vector (pCLNDX Retrovirus expression Vector [NBP2-29540]) with lentiviral vector? I have been using the system of plasmids carrying the gag-pol, rev, GFP-lentivirus and vsv-g (to infect human cells). Can I use the PCL-Eco to infect mouse cells instead of vsv-g?

    A: Unfortunately, we have not tested lenti-virus in this packaging kit so I cannot tell you if it will work or not. There are lots of packaging kits on the market for lenti-virus so if you take a look maybe you can find one that will be certain to work. We, however, do not have a lenti-virus kit.

  • Q: What is the packaging size limitation on Eco Retrovirus Packaging Vector NBP2-29540?

    A: The combined size of two inserts should not be more than 4 kb.

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