Lyophilized OneStep RT-qPCR Master Mix

The Lyophilized OneStep RT-qPCR Master Mix is an optimized complete system for reproducible one-step, reverse transcription (RT) qPCR experiments using hydrolysis probes. Removal of a separate reverse transcription step reduces handling errors, and greatly reduces the time taken to obtain results. The Lyophilized OneStep RT-qPCR Master Mix is stable at ambient temperatures and can be conveniently shipped and stored at room temperature. The Lyophilized OneStep RT-qPCR Master Mix contains a thermo-stable TAQ Polymerase and MMLV as well as buffer, dNTPs, MgCl₂ and stabilizers at concentrations optimized for the enzymes. Once resuspended, only the template RNA and primer and probe mix are required to complete the experimental set up for a single tube reaction.

Lyophilized OneStep RT-qPCR Master Mix contains sufficient reagent for 150 reactions.

For further information regarding preparation, storage and use of the Lyophilized OneStep RT-qPCR Master Mix, please view the product protocol.