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Exosome Standards (Human Plasma)

Catalog # NBP3-11686 | Novus Biologicals a Bio-Techne Brand
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NBP3-11686-180ug
NBP3-11686-200ug
NBP3-11686-600ug
NBP3-11686-60ug

Key Product Details

Applications

ELISA, Flow Cytometry, Microscopy, N/A, Western Blot

Product Summary for Exosome Standards (Human Plasma)

Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications including: Assay calibration, Spike-in control for exosome quantification, Protein marker analysis for different techniques such as Western Blot and Flow Cytometry, Extraction and analysis of exosomal RNA and DNA, Standardized positive controls for evaluating immunocapture performance.

Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10). Store lyophilized exosomes up to 3 years at 4C (Expire date indicated on the label). For reconstituted exosomes store at -20C for up to one month or at -80C for up to 6 months. Avoid repeated freeze-and-thraw cycles.

Scientific Data Images for Exosome Standards (Human Plasma)

Western Blot: Exosome Standards (Human Plasma) [NBP3-11686] - Marker detection by Western Blotting. Reconstituted Exosomes can be directly lysed in Laemmli buffer, then loaded on the Electrophoresis gel. Recommended quantity: 10-20 ug per line.
Nucleic Acid Extraction: Exosome Standards (Human Plasma) [NBP3-11686] - Profiling of Exosome associated nucleic acids. Reconstituted Exosomes can be used for profiling nucleic acids (RNAs and DNA) biomarkers. The lysis buffer can be added directly to the Exosome preparation. Recommended quantity: 20-50 ug perreaction.

Formulation, Preparation, and Storage

Preparation Method

Isolation involves ultracentrifugation and microfiltration. Exosomes are quantified and validated for protein content and particle number by Nanoparticles Tracking Analysis. Lyophilization does not alter stability of exosome proteins and nucleic acids.

Formulation

Lyophilized from human biofluids of healthy donors

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Reconstitution Instructions

Add deionized water, 100 ul for Standard 100 ug and 30 ul for Standard 30 ug, to get a final concentration of 1 mg/mL. Resuspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 seconds.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. After reconstitution store at -70C.

Background: Exosome Standards (Human Plasma)

Exosomes are small endosome derived lipid nanoparticles (30-150nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Product Documents for Exosome Standards (Human Plasma)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Exosome Standards (Human Plasma)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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