Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71710
Key Product Details
Applications
Chromatin Immunoprecipitation
Kit Type
Kit (ChIP)
Product Summary for Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
This ChIP kit is specific for Histone H3 acetylated Lysine 4, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS). This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately.
This kit includes a full size vial of acetyl Lys4 antibody (NB21-1024).
This kit includes a full size vial of acetyl Lys4 antibody (NB21-1024).
Scientific Data Images for Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] ChIP Kit [NBP1-71710] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] ChIP Kit [NBP1-71710] - Chromata ChIP Histone H3 [ac Lys4] Kit [NBP1-71710] - qRT-PCR standard curve generated from a serial dilution of sheared HeLa chromatin.
Chromatin Immunoprecipitation: Chromata ChIP Histone H3 [ac Lys4] ChIP Kit [NBP1-71710] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Kit Contents for Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
- 10X Glycine (30 mL)
- 5M NaCl (1 mL)
- DNA Binding Buffer (5 mL - add 20 mL EtOH)
- DNA Elution Buffer (2 mL)
- H3 acetyl Lys4 Antibody (0.05 mg) - NB21-1024
- H3 K4Me3 Positive Control Antibody (0.025 mg) - NB21-1023SS
- Human AFM Negative Control Primer Pair at 10 uM (0.5 mL) - NBP1-71653
- Human RPL30 Positive Control Primer Pair at 10 uM (0.5 mL) - NBP1-71651
- IP Dilution Buffer (2 x 25 mL)
- IP Elution Buffer (7 mL)
- IP Wash Buffer 1 (15 mL)
- IP Wash Buffer 2 (15 mL)
- IP Wash Buffer 3 (15 mL)
- IP Wash Buffer 4 (15 mL)
- Protein A/G Magnetice Beads (0.8 mL)
- Proteinase K (0.06 mL)
- Purification Columns (30 EA)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage of components varies. See protocol for specific instructions.
Background: Chromata ChIP Histone H3
Alternate Names
H3K4ac
Additional Chromata ChIP Histone H3 Products
Product Documents for Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
Product Specific Notices for Chromata ChIP Histone H3 [ac Lys4] Kit (ChIP)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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