Rat Methylcellulose Complete Media Without Epo

Complete media for the differentiation and enumeration of rat colony-forming myeloid progenitors (CFU-GM) and for the identification of Epo-independent erythropoiesis.

Why use R&D Systems Rat Methylcellulose Complete Media Without Epo for Colony Forming Cell Assays?

Colony forming cell (CFC) assays, which are used to enumerate and quantify multi-potent and single lineage hematopoietic progenitors, can be time consuming and laborious.

Successful growth and enumeration of cell colonies is dependent on factors such as accurate cell counts, the presence of growth factors and/or cytokines, adequate humidity, and the use of high quality media. R&D Systems offers Rat Methylcellulose Complete Media Without Epo, which contains premium quality recombinant rat cytokines to support the optimal growth and enumeration of colony-forming myeloid progenitors (CFU-GM) of rat origin. The absence of Epo makes this media useful for the identification of Epo-independent stimulators of erythropoiesis.

R&D Systems Rat Methylcellulose Complete Media Without Epo:

• Can be used to identify Epo-independent stimulators of erythropoiesis.
• Includes premium quality rat recombinant cytokines to support growth of CFU-GM.
• Optical clarity facilitates colony identification.
• High lot-to-lot consistency decreases variation.
• Does not require addition of serum or cytokines.

• 100 mL of Rat Methylcellulose Complete Media Without Epo.

Stability and Storage

Rat Methylcellulose Complete Media Without Epo should be stored at ≤-20 °C upon receipt. Storage at 2 °C to 8 °C is not recommended.

Precautions

• The acute and chronic effects of overexposure to this media are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling this media.

Limitations

• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• The reagent should not be used beyond the expiration date indicated on the vial labels.
• The media is optimized to assay rat hematopoietic progenitors and is ineffective with human hematopoietic progenitors.
• Derivation of human hematopoietic progenitors from different individuals may cause results to vary.

Rat Hematopoietic Colony Formation Using the Methylcellulose-based Colony Forming Cell Assay. A single cell suspension of rat bone marrow cells was resuspended in Rat Methylcellulose Complete Media Without Epo (Catalog # HSC012) and used in the Colony Forming Cell (CFC) assay. Colonies were viewed under light microscope after an 8-12 day incubation. A. Colony forming unit-granulocyte, macrophage (CFU-GM) are progenitors that give rise to colonies containing a heterogeneous population of macrophages and granulocytes. B and C. Colony forming unit- macrophage (CFU-M) are clonogenic progenitors of macrophages that give rise to a homogenous population of macrophages.

Rat Methylcellulose Stock and Base Media

Complete Rat Methylcellulose Media

*Base media and stock solutions do not contain cytokines and will not support colony growth unless conditioned media, cytokines, or other culture supplements are added.

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte­nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu­ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple­tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.