Human/Mouse/Rat Neural Lineage Functional Identification Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Reagents Provided
Reagents supplied in the Human/Mouse/Rat Neural Progenitor Cell Functional Identification Kit (Catalog # SC028):
- Neural Maintenance Supplement (500X)
- Neural Differentiation Supplement (100X)
- Bovine Fibronectin (100X)
- NPC Marker: Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody
- Astrocyte Marker: Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody
- Neuron Marker: Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody
- Oligodendrocyte Marker: Mouse Anti-Oligodendrocyte Marker O4 Monoclonal Antibody
Note: The quantity of neural differentiation supplement in this kit is sufficient to make 50 mL of media for NPC expansion and 100 mL of medium for NPC differentiation. This is enough medium for the differentiation of two 24-well plates.
Other Supplies Required
Reagents
- N-2 MAX Media Supplement (Catalog # AR009)
- Poly-L-Ornithine
- Phosphate buffered saline (PBS)
- Penicillin-Streptomycin (100X)
- Bovine serum albumin (BSA)
- D-MEM/F-12 (1X)
- Glucose
- L-Glutamine
- Sodium Bicarbonate (NaHCO3)
- Trypan blue
- 95% Ethanol
- Deionized or distilled water
Materials
- Human, mouse, or rat NPCs
- 24-well culture plates
- 12 mm coverslips
- 15 mL centrifuge tubes
- Pipettes and pipette tips
- Serological pipettes
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
- Hemocytometer
- Inverted microscope
- 37 °C water bath
- 0.2 µm filter unit, 250 mL
Procedure Overview
![]() Prepare Poly-L-Ornithine- and Fibronectin-coated coverslips. | |||
![]() Plate 0.5 -1.0 x 106 NPCs in media containing Neural Maintenance Supplement. Culture cells to 50% confluency. | |||
![]() Replace with fresh media after 24 hours. Culture cells for 48 hours after initial plating. | |||
ICC detection of Nestin to identify NPCs. | |||
![]() Astrocyte Differentiation | ![]() Neuron Differentiation | ![]() Oligodendrocyte Differentiation | |
---|---|---|---|
Day 1 | Replace media with Neural Differentiation Media. | Replace media with Neural Differentiation Media. | Replace media with Neural Differentiation Media. |
Day 4 | Repeat media change every 3 days. | Repeat media change every 3 days. | Repeat media change every 3 days. |
Day 7-10 | ICC detection of GFAP. | ICC detection of beta-III Tubulin. | ICC detection of Oligodendrocyte Marker O4. |
Citations for Human/Mouse/Rat Neural Lineage Functional Identification Kit (2)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat Neural Lineage Functional Identification Kit include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCL Xiao et al. (2017), Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro Int J Mol Sci, 18(8).
PMID: 28800076 -
Species: Human
Sample Types: Whole Cells
Donovan et al. (2012), A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles. Transl Oncol, 5(3):141-54.
PMID: 22741033
There are no citations that match your criteria.
FAQs for Human/Mouse/Rat Neural Lineage Functional Identification Kit
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Can Human/Mouse/Rat Neural Lineage Functional Identification Kit (Catalog # SC028) be used with both embryonic and adult neural progenitor cells?
The Human/Mouse/Rat Neural Lineage Functional Identification Kit has been tested with embryonic neural progenitor cells. The kit should also work with adult neural progenitor cells, as adult cells have similar growth conditions.