Hematoxylin
Tocris Bioscience, part of Bio-Techne | Catalog # 5222
Histologic stain; used to stain cell nuclei
Discontinued Product
5222 has been discontinued.
View all Histology Stains products.
Key Product Details
Description
Histologic stain; used to stain cell nuclei
Product Description
Hematoxylin is a dye used to stain cell nuclei. Hematoxylin is commonly combined with eosin for H&E histological staining and is a component of the Papanicolaou stain used in cytology. Hematoxylin can be used with other fixatives to stain muscle striations, fibrin, myelin and elastin. Can also be used for H&E staining of intact tissues.Product Specifications
Molecular Weight
302.28
Formula
C16H14O6
Storage
Store at RT
Chemical Name
(6aS,11bR)-7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol
CAS Number
517-28-2
PubChem ID
442514
InChI Key
WZUVPPKBWHMQCE-XJKSGUPXSA-N
SMILES
OC1=C(O)C=C(C[C@]3(O)[C@]([H])2C4=C(C(O)=C(O)C=C4)OC3)C2=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| Ethanol | 0.91 | 3 |
Preparing Stock Solutions
for Hematoxylin
The following data is based on the product molecular weight 302.28
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.03 mM | 110.27 mL | 551.37 mL | 1,102.73 mL |
| 0.15 mM | 22.05 mL | 110.27 mL | 220.55 mL |
| 0.3 mM | 11.03 mL | 55.14 mL | 110.27 mL |
| 1.5 mM | 2.21 mL | 11.03 mL | 22.05 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Li Hematoxylin and eosin staining of intact tissues via delipidation and ultrasound, Sci.Rep. 2018 PMID: 30115962
Product Specific Notices for Hematoxylin
For research use only
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