MRT 10
Tocris Bioscience, part of Bio-Techne | Catalog # 4585
Smoothened (Smo) antagonist
Discontinued Product
4585 has been discontinued.
View all Smoothened Receptor Antagonists products.
Key Product Details
Description
Smoothened (Smo) antagonist
Product Description
MRT 10 is a smoothened (Smo) receptor antagonist (IC50 = 0.5 μM in HEK293 cells transiently expressing mouse Smo). Inhibits ShhN-induced Gli luciferase reporter activity in Shh-light 2 cells (IC50 = 2.5 μM); acts as an inverse agonist during nontranscriptional Hedgehog pathway activation (IC50 = 2.5 μM in HEK293 cells).Product Specifications
Molecular Weight
465.52
Formula
C24H23N3O5S
Storage
Store at +4°C
Purity
≥98%
Chemical Name
N-[[[3-Benzoylamino)phenyl]amino]thioxomethyl]-3,4,5-trimethoxybenzamide
CAS Number
330829-30-6
PubChem ID
1139102
InChI Key
KVQVEZQDNHMQJV-UHFFFAOYSA-N
SMILES
O=C(NC(NC2=CC(NC(C3=CC=CC=C3)=O)=CC=C2)=S)C1=CC(OC)=C(OC)C(OC)=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 46.55 | 100 |
Preparing Stock Solutions
for MRT 10
The following data is based on the product molecular weight 465.52
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.15 mL | 10.74 mL | 21.48 mL |
| 5 mM | 0.43 mL | 2.15 mL | 4.30 mL |
| 10 mM | 0.21 mL | 1.07 mL | 2.15 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.43 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Manetti Virtual screening-based discovery and mechanistic characterization of the acylthiourea MRT-10 family as smoothened antagonists. Mol.Pharmacol. 2010 PMID: 20664000
- Solinas Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity. J.Med.Chem. 2012 PMID: 22268551
Product Specific Notices for MRT 10
For research use only
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