VU 0240551

Name: VU 0240551
Brand: Tocris Bioscience
SKU: 3888
Price: 227 USD

Catalog # 3888 | Tocris Bioscience a Bio-Techne Brand

Citations (8)

COA / SDS

KCC2 inhibitor

Key Product Details

Description:	KCC2 inhibitor
Chemical Name:	N-(4-Methyl-2-thiazolyl)-2-[(6-phenyl-3-pyridazinyl)thio]acetamide
Purity:	≥99% (HPLC)
Molecular Weight:	342.43

View Full Technical Details for VU 0240551

Biological Activity for VU 0240551

VU 0240551 is an inhibitor of the neuronal K-Cl cotransporter, KCC2 (IC₅₀ = 560 nM for K⁺ uptake assay in KCC2-overexpressing cells). Exhibits selectivity over the Na-K-2Cl cotransporter, NKCCl. Also inhibits hERG and L-type Ca²⁺ channels.

Technical Data for VU 0240551

M. Wt	342.43
Formula	C₁₆H₁₄N₄OS₂
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	893990-34-6
PubChem ID	7211972
InChi Key	WJRWSLORVIHRNX-UHFFFAOYSA-N
Smiles	O=C(NC3=NC(C)=CS3)CSC(C=C2)=NN=C2C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for VU 0240551

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	34.24	100
	ethanol	8.56	25

Preparing Stock Solutions for VU 0240551

The following data is based on the product molecular weight 342.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.92 mL	14.60 mL	29.20 mL
5 mM	0.58 mL	2.92 mL	5.84 mL
10 mM	0.29 mL	1.46 mL	2.92 mL
50 mM	0.06 mL	0.29 mL	0.58 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for VU 0240551

References are publications that support the biological activity of the product.

Deisz Effects of VU0240551, a novel KCC2 antagonist, and DIDS on chloride homeostasis of neocortical neurons from rats and humans. Neuroscience 2014 PMID: 25086309
Delpire Further optimization of the K-Cl cotransporter KCC2 antagonist ML077: development of a highly selective and more potent in vitro probe. Bioorg.Med.Chem.Lett. 2012 PMID: 22727639
Delpire Small-molecule screen identifies inhibitors of the neuronal K-Cl cotransporter KCC2. Proc.Natl.Acad.Sci.USA 2009 PMID: 19279215

Citations for VU 0240551 (8)

Showing 1 - 8 of 8 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for VU 0240551 include:

Marie-Pascale et al. (2021) Enhancing KCC2 activity decreases hyperreflexia and spasticity after chronic spinal cord injury. Exp Neurol. 338:113605
PMID: 33453210
Alfredo et al. (2020) Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain. Nat Commun. 11:869
PMID: 32054836
Ferando et al. (2016) Diminished KCC2 confounds synapse specificity of LTP during senescence. Nat Neurosci. 19:1197
PMID: 27500406
Yi et al. (2016) Potentiation of Synaptic GluN2B NMDAR Currents by Fyn Kinase Is Gated through BDNF-Mediated Disinhibition in Spinal Pain Processing. Cell Rep. 17:2753-2765
PMID: 27926876
Lavertu et al. (2014) Enhancing K-Cl co-transport restores normal spinothalamic sensory coding in a neuropathic pain model. Brain. 137:724
PMID: 24369380
Gagnon et al. (2013) Chloride extrusion enhancers as novel therapeutics for neurological diseases. Nat Med. 19:1524
PMID: 24097188
Haam et al. (2012) GABA is excitatory in adult vasopressinergic neuroendocrine cells. J Neurosci. 32:572
PMID: 22238092
Doyon et al. (2011) Efficacy of synaptic inhibition depends on multiple, dynamically interacting mechanisms implicated in chloride homeostasis. Purinergic Signal. 7:e1002149
PMID: 21931544

There are no citations that match your criteria.

Showing 1 - 8 of 8 citations Showing All

Product Documents for VU 0240551

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.