SEA 0400

Name: SEA 0400
Brand: Tocris Bioscience
SKU: 6164

Tocris Bioscience a Bio-Techne Brand

Catalog # 6164

Citations (1)

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Key Product Details

Description:	Potent Na⁺/Ca²⁺ exchanger (NCX) inhibitor
Chemical Name:	2-[4-[(2,5-Difluorophenyl)methoxy]phenoxy]-5-ethoxybenzenamine
Purity:	≥98% (HPLC)
Molecular Weight:	371.38

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Biological Activity for SEA 0400

SEA 0400 is a potent Na⁺/Ca²⁺ exchanger (NCX) inhibitor. Inhibits NCX activity in rat astrocytes, microglia and cultured neurons, canine cardiac sarcolemmal vesicles and rat cardiomyocytes (IC₅₀ values are 5 and 8.3, 33, 90 and 92 nM, respectively). Reduces infarct volumes and exhibits protective effects against myocardial ischemia in rats.

Compound Libraries for SEA 0400

SEA 0400 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SEA 0400

M. Wt	371.38
Formula	C₂₁H₁₉F₂NO₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	223104-29-8
PubChem ID	644100
InChi Key	YSUBLPUJDOWYDP-UHFFFAOYSA-N
Smiles	CCOC1=CC(N)=C(C=C1)OC2=CC=C(C=C2)OCC3=C(C=CC(F)=C3)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SEA 0400

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	37.14	100
	ethanol	37.14	100

Preparing Stock Solutions for SEA 0400

The following data is based on the product molecular weight 371.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.69 mL	13.46 mL	26.93 mL
5 mM	0.54 mL	2.69 mL	5.39 mL
10 mM	0.27 mL	1.35 mL	2.69 mL
50 mM	0.05 mL	0.27 mL	0.54 mL

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References for SEA 0400

References are publications that support the biological activity of the product.

Christ Block of Na(⁺)/Ca(²⁺) exchanger by SEA0400 in human right atrial preparations from patients in sinus rhythm and in atrial fibrillation. Eur.J.Pharmacol. 2016 PMID: 27373849
Karashima Involvement of Na⁺-Ca²⁺ exchanger in cAMP-mediated relaxation in mice aorta: evaluation using transgenic mice. Br.J.Pharmacol. 2007 PMID: 17220909
Takahashi Protective effects of SEA0400, a novel and selective inhibitor of the Na⁺/Ca²⁺ exchanger, on myocardial ischemia-reperfusion injuries. Eur.J.Pharmacol. 2003 PMID: 12498920

Citations for SEA 0400 (1)

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Citations are publications that use Bio-Techne products. Selected citations for SEA 0400 include:

Neubert et al. (2020) NCX1 Represents an Ionic Na⁺ Sensing Mechanism in Macrophages. PLoS Biol. 18
PMID: 32569301

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